The phosphorylation levels of ERK1/2 and p38 were suppressed by the inhibiton of PAX6 expression.

<p>Western-blot analysis of A549, A549 NC, A549 PAX6 KD, H1299, H1299NC, H1299 PAX6 KD with antibodies to ERK1/2 (A), p38(B) and their phosphorylated forms were shown in the figure. GAPDH and β-actin was used as internal loading controls respectively. ERK1/2 and p38 levels were normalized by GAPDH and β-actin respectively. Data are expressed as mean ±SEM. All the experiments were repeated three times. *P <0.05, **P <0.01.