Cell cycle progression is altered in DCT knockdown cells compared to control cells.

<p>(A) Cell lysates from control and DCT knockdown cells were harvested and subjected to western blotting on the day of the knockdown. pRb (Ser807/811) and actin levels are shown for three independent DCT knockdown experiments. (B) pRb band intensities of each lane were quantified and normalized first against the corresponding actin measurements and then against the normalized pRb levels in control samples. The bar graph shows the normalized pRb levels of DCT knockdown cells compared to their controls. (C) Representative cell cycle profiles for DCT knockdown and control cells as analyzed and visualized by FlowJo v10. (D) Cell cycle profiles of DCT knockdown and control cells were analyzed by PI staining and flow cytometer. The bar graph shows the mean value of the percentage of cells in G0/G1 phase for three independent experiments with six replicates each (one tailed t-test p = 0.0008).