RAD6 regulates the expression of cyclin D1 in both the mRNA and protein level.

<p>(<b>A</b>) HL-7702 cells transfected with an empty control or Myc-RAD6 expressing plasmids were lysed and subjected to RT-PCR and western blot assays. (<b>B</b>) HL-7702 cells transfected with control or RAD6 siRNAs were lysed and subjected to RT-PCR and western blot assays. (<b>C</b>) HL-7702 cells transfected with an empty control and Myc-RAD6 plasmids together with or without CCND1 siRNA were harvested and stained with PI, and then cells were subjected to cell cycle assay. The quantification of the cell cycle assay is shown. (<b>D</b>) Soft agar colony formation assays were performed using control, or RAD6 overexpressing, or RAD6 overexpressing together with CCND1 knocking down HL-7702 cells. (<b>E</b>) HL-7702 cells used in the above assays were lysed and subjected to western blot analyses with antibodies against CCND1, Myc-tag and Actin as indicated.