Regulation of <i>cyclin D1, p53, and p21</i> mRNA and protein expression of in MCF-7p14ARF.

<p>Cells were treated with IPTG, ICI 182780 (ICI), or E2 for 12 h. Top panel: relative gene expression of <b>A... </b><i>CCDN1</i>, <b>B... </b><i>TP53</i> and <b>C... </b><i>CDKN1A</i> mRNA quantified by qRT-PCR and relative expression normalised to control <i>ACTB. Columns,</i> Data represents the mean ± SEM of three separate experiments (** P<0.001. number = 9). Bottom panel: representative Western blots of <b>A.</b> cyclin D1, <b>B.</b> p53, and <b>C.</b> p21. Relative levels of protein are shown above each lane. β-actin was used as a control. The representative images are all from the same sample set, run on the same gel and Western blot.