Western blot analysis of cell cycle related proteins of MCF-7 cells treated with 300 µg/ml PRME.

The expression of cell cycle related proteins in vitro.

Zhe Chen (196097)
Ze-Zhang Tao (498022)
Shi-Ming Chen (498023)
Chen Chen (6544)
Fen Li (337013)
Bo-kui Xiao (498024)

December 2013

Key proteins related to cycle regulation were detected by WB. As I3C concentration increased, cyc...

Restriction point and metabolic checkpoint arrest lead to differential patterns of cell cycle regulator expression and phosphorylation.

Mahesh Saqcena (447633)
Deepak Menon (447634)
Deven Patel (376312)
Suman Mukhopadhyay (190415)
Victor Chow (447635)
David A. Foster (447636)

August 2013

(A) Cells were plated at 30% confluence in 10-cm plates in DMEM containing 10% FBS. After ...

Immunoblotting analysis of protein levels.

Carolina Vizcaíno (246726)
Luz-Elena Núñez (169843)
Francisco Morís (169876)
José Portugal (30112)

August 2014

(A) Western blots showing changes in protein levels in A2780 cells treated with 80 nM DIG-MSK for...

Western blot analysis of apoptosis related proteins of MCF-7 cells treated with 300 µg/ml PRME.

Nikhil Baban Ghate (498102)
Dipankar Chaudhuri (498103)
Rhitajit Sarkar (498104)
Albert L. Sajem (498105)
Sourav Panja (498106)
Jayashree Rout (498107)
Nripendranath Mandal (498108)

December 2013

Graphs adjoining the blots represent the expression levels of corresponding proteins for indicate...

Western blot analysis of canonical cell cycle proteins.

Emily M. Astarita (11250513)
Sara M. Maloney (11250516)
Camden A. Hoover (11250519)
Bronwyn J. Berkeley (11250522)
Monica K. VanKlompenberg (11250525)
T. Murlidharan Nair (11250528)
Jenifer R. Prosperi (11250531)

August 2021

Untreated or PTX treated lysates were probed for expression of canonical cell cycle proteins. (A) Re...

Analysis by flow cytometry of cell cycle profiles.

Ana Galán-Cobo (795784)
Reposo Ramírez-Lorca (222662)
Ana Serna (795785)
Miriam Echevarría (164223)

September 2015

(A). Cell cycle profiles showing the distribution of cells in the different phases of the cell cy...

Western blot analysis of phosphorylated CDK1 (Thr-161), phosphorylated CDK1 (Tyr-15), CDK1, cyclin B1, phosphorylated-CDC25C (Thr-48), CDC25C, phosphorylated-Histone H3 (Ser-10), Histone H3, phosphorylated-CHK2 (Ser-19), CHK2, phosphorylated p53 (Ser-15), p53, and GAPDH (A), and phosphorylated BCL-2 (Ser-70), BCL-2, BCL-XL, BAG3, phosphorylated MCL-1 (Ser-159/Thr-169), MCL-1, BAK, reduction in the electrophoretic mobility of the BIM isoforms (BIMEL and BIML), caspase-9, PARP, and GAPDH (B) in J/Neo cells treated with NOC, 2-MeO-E2, or CPT in the absence and presence of CMEP-NQ for 17 h.

Do Youn Jun (1429327)
Won Young Jang (3993092)
Ki Yun Kim (5807312)
Mi Hee Woo (1429309)
Young Ho Kim (121751)

October 2018

After pretreatment with 7.5 μM CMEP-NQ for 1 h, J/Neo cells (5 × 105/mL) were treated with 0.3 μM NO...

Western blot analysis of (A) kinase-related protein expression and (B) cell cycle-related protein expression in HEY PGCCs alone, HEY PGCCs with budding daughter cells, control HEY cells, SKOv3 PGCCs alone, and control SKOv3 cells.

Shiwu Zhang (85788)
Imelda Mercado-Uribe (484738)
Samir Hanash (55377)
Jinsong Liu (6516)

November 2013

Western blot analysis of (A) kinase-related protein expression and (B) cell cycle-related protein...

Cyclin A, Cyclin B1and Cdc2 evaluation by western blot.

Maia Cabrera (705603)
Natalia Gomez (705602)
Federico Remes Lenicov (337979)
Emiliana Echeverría (794349)
Carina Shayo (705604)
Albertina Moglioni (547262)
Natalia Fernández (5662648)
Carlos Davio (705605)

September 2015

(A)Total lysates of cells exposed to 10 (HL60) and 20 μM (U937, KG1a and Jurkat) T44Bf for 15 and...

Western Blot analysis of cell cycle regulating (fig. 2A) and cell signaling proteins (fig. 2B) in tumor tissue from drug-sensitive (responders) versus drug-resistant mice (non-responders).

Eva Juengel (287273)
Jasmina Makarević (6260)
Igor Tsaur (285993)
Georg Bartsch (287275)
Karen Nelson (287278)
Axel Haferkamp (287281)
Roman A. Blaheta (287284)

February 2013

Control tissue specimens were taken from untreated animals. Cell lysates (50 µg) were subjected t...

Western blot analysis of cellular fractions.

Emily M. Astarita (11250513)
Sara M. Maloney (11250516)
Camden A. Hoover (11250519)
Bronwyn J. Berkeley (11250522)
Monica K. VanKlompenberg (11250525)
T. Murlidharan Nair (11250528)
Jenifer R. Prosperi (11250531)

August 2021

Protein lysates from CTL, PTX, or nocodazole (NOC) treated cells were fractionated and then probed w...

Real time quantitative PCR and western blot analyses of candidate markers of the SP in MM cells.

Miho Nara (385066)
Kazuaki Teshima (385067)
Atsushi Watanabe (64183)
Mitsugu Ito (385068)
Keiko Iwamoto (385069)
Atsushi Kitabayashi (385070)
Masaaki Kume (385071)
Yoshiaki Hatano (385072)
Naoto Takahashi (385073)
Shinsuke Iida (385074)
Kenichi Sawada (385075)
Hiroyuki Tagawa (385076)

March 2013

(A). Real time quantitative PCR of cell cycle and mitosis related genes (CCNB1,CDC2, CDC20, CD...

Comparative analysis of p14ARF transcriptional and post-transcriptional regulation of cyclin D1, p53 and p21 by p14ARF.

Eileen M. McGowan (146504)
Nham Tran (146510)
Nikki Alling (146519)
Daniel Yagoub (146528)
Lisa M. Sedger (146537)
Rosetta Martiniello-Wilks (146544)

July 2012

MCF-7p14ARF 4a cells were treated with IPTG (lanes, 2, 4, 5, 8) or control (lanes 1, 3, 5, 7). Ce...

Western blot analysis of APC expression in parental and APC shRNA1 cells.

Emily M. Astarita (11250513)
Sara M. Maloney (11250516)
Camden A. Hoover (11250519)
Bronwyn J. Berkeley (11250522)
Monica K. VanKlompenberg (11250525)
T. Murlidharan Nair (11250528)
Jenifer R. Prosperi (11250531)

August 2021

(A) Untreated cell lysates were probed for APC expression and representative western blot is shown. ...

Cell cycle regulation of IFT proteins.

Christopher R. Wood (185375)
Zhaohui Wang (144332)
Dennis Diener (185379)
James Matt Zones (185381)
Joel Rosenbaum (185388)
James G. Umen (185393)

February 2012

(A) Top panel is Coomassie Blue (CB) stained gel of total protein from indicated times. Equal pro...

The expression of cell cycle related proteins in vitro.

Zhe Chen (196097)
Ze-Zhang Tao (498022)
Shi-Ming Chen (498023)
Chen Chen (6544)
Fen Li (337013)
Bo-kui Xiao (498024)

December 2013

Key proteins related to cycle regulation were detected by WB. As I3C concentration increased, cyc...

Restriction point and metabolic checkpoint arrest lead to differential patterns of cell cycle regulator expression and phosphorylation.

Mahesh Saqcena (447633)
Deepak Menon (447634)
Deven Patel (376312)
Suman Mukhopadhyay (190415)
Victor Chow (447635)
David A. Foster (447636)

August 2013

(A) Cells were plated at 30% confluence in 10-cm plates in DMEM containing 10% FBS. After ...

Immunoblotting analysis of protein levels.

Carolina Vizcaíno (246726)
Luz-Elena Núñez (169843)
Francisco Morís (169876)
José Portugal (30112)

August 2014

(A) Western blots showing changes in protein levels in A2780 cells treated with 80 nM DIG-MSK for...

Western blot analysis of apoptosis related proteins of MCF-7 cells treated with 300 µg/ml PRME.

Nikhil Baban Ghate (498102)
Dipankar Chaudhuri (498103)
Rhitajit Sarkar (498104)
Albert L. Sajem (498105)
Sourav Panja (498106)
Jayashree Rout (498107)
Nripendranath Mandal (498108)

December 2013

Graphs adjoining the blots represent the expression levels of corresponding proteins for indicate...

Western blot analysis of canonical cell cycle proteins.

Emily M. Astarita (11250513)
Sara M. Maloney (11250516)
Camden A. Hoover (11250519)
Bronwyn J. Berkeley (11250522)
Monica K. VanKlompenberg (11250525)
T. Murlidharan Nair (11250528)
Jenifer R. Prosperi (11250531)

August 2021

Untreated or PTX treated lysates were probed for expression of canonical cell cycle proteins. (A) Re...

Analysis by flow cytometry of cell cycle profiles.

Ana Galán-Cobo (795784)
Reposo Ramírez-Lorca (222662)
Ana Serna (795785)
Miriam Echevarría (164223)

September 2015

(A). Cell cycle profiles showing the distribution of cells in the different phases of the cell cy...

Western blot analysis of phosphorylated CDK1 (Thr-161), phosphorylated CDK1 (Tyr-15), CDK1, cyclin B1, phosphorylated-CDC25C (Thr-48), CDC25C, phosphorylated-Histone H3 (Ser-10), Histone H3, phosphorylated-CHK2 (Ser-19), CHK2, phosphorylated p53 (Ser-15), p53, and GAPDH (A), and phosphorylated BCL-2 (Ser-70), BCL-2, BCL-XL, BAG3, phosphorylated MCL-1 (Ser-159/Thr-169), MCL-1, BAK, reduction in the electrophoretic mobility of the BIM isoforms (BIMEL and BIML), caspase-9, PARP, and GAPDH (B) in J/Neo cells treated with NOC, 2-MeO-E2, or CPT in the absence and presence of CMEP-NQ for 17 h.

Do Youn Jun (1429327)
Won Young Jang (3993092)
Ki Yun Kim (5807312)
Mi Hee Woo (1429309)
Young Ho Kim (121751)

October 2018

After pretreatment with 7.5 μM CMEP-NQ for 1 h, J/Neo cells (5 × 105/mL) were treated with 0.3 μM NO...

Western blot analysis of (A) kinase-related protein expression and (B) cell cycle-related protein expression in HEY PGCCs alone, HEY PGCCs with budding daughter cells, control HEY cells, SKOv3 PGCCs alone, and control SKOv3 cells.

Shiwu Zhang (85788)
Imelda Mercado-Uribe (484738)
Samir Hanash (55377)
Jinsong Liu (6516)

November 2013

Western blot analysis of (A) kinase-related protein expression and (B) cell cycle-related protein...

Cyclin A, Cyclin B1and Cdc2 evaluation by western blot.

Maia Cabrera (705603)
Natalia Gomez (705602)
Federico Remes Lenicov (337979)
Emiliana Echeverría (794349)
Carina Shayo (705604)
Albertina Moglioni (547262)
Natalia Fernández (5662648)
Carlos Davio (705605)

September 2015

(A)Total lysates of cells exposed to 10 (HL60) and 20 μM (U937, KG1a and Jurkat) T44Bf for 15 and...

Western Blot analysis of cell cycle regulating (fig. 2A) and cell signaling proteins (fig. 2B) in tumor tissue from drug-sensitive (responders) versus drug-resistant mice (non-responders).

Eva Juengel (287273)
Jasmina Makarević (6260)
Igor Tsaur (285993)
Georg Bartsch (287275)
Karen Nelson (287278)
Axel Haferkamp (287281)
Roman A. Blaheta (287284)

February 2013

Control tissue specimens were taken from untreated animals. Cell lysates (50 µg) were subjected t...

Western blot analysis of cellular fractions.

Emily M. Astarita (11250513)
Sara M. Maloney (11250516)
Camden A. Hoover (11250519)
Bronwyn J. Berkeley (11250522)
Monica K. VanKlompenberg (11250525)
T. Murlidharan Nair (11250528)
Jenifer R. Prosperi (11250531)

August 2021

Protein lysates from CTL, PTX, or nocodazole (NOC) treated cells were fractionated and then probed w...

Real time quantitative PCR and western blot analyses of candidate markers of the SP in MM cells.

Miho Nara (385066)
Kazuaki Teshima (385067)
Atsushi Watanabe (64183)
Mitsugu Ito (385068)
Keiko Iwamoto (385069)
Atsushi Kitabayashi (385070)
Masaaki Kume (385071)
Yoshiaki Hatano (385072)
Naoto Takahashi (385073)
Shinsuke Iida (385074)
Kenichi Sawada (385075)
Hiroyuki Tagawa (385076)

March 2013

(A). Real time quantitative PCR of cell cycle and mitosis related genes (CCNB1,CDC2, CDC20, CD...

Comparative analysis of p14ARF transcriptional and post-transcriptional regulation of cyclin D1, p53 and p21 by p14ARF.

Eileen M. McGowan (146504)
Nham Tran (146510)
Nikki Alling (146519)
Daniel Yagoub (146528)
Lisa M. Sedger (146537)
Rosetta Martiniello-Wilks (146544)

July 2012

MCF-7p14ARF 4a cells were treated with IPTG (lanes, 2, 4, 5, 8) or control (lanes 1, 3, 5, 7). Ce...

Western blot analysis of APC expression in parental and APC shRNA1 cells.

Emily M. Astarita (11250513)
Sara M. Maloney (11250516)
Camden A. Hoover (11250519)
Bronwyn J. Berkeley (11250522)
Monica K. VanKlompenberg (11250525)
T. Murlidharan Nair (11250528)
Jenifer R. Prosperi (11250531)

August 2021

(A) Untreated cell lysates were probed for APC expression and representative western blot is shown. ...

Cell cycle regulation of IFT proteins.

Christopher R. Wood (185375)
Zhaohui Wang (144332)
Dennis Diener (185379)
James Matt Zones (185381)
Joel Rosenbaum (185388)
James G. Umen (185393)

February 2012

(A) Top panel is Coomassie Blue (CB) stained gel of total protein from indicated times. Equal pro...

The expression of cell cycle related proteins in vitro.

Zhe Chen (196097)
Ze-Zhang Tao (498022)
Shi-Ming Chen (498023)
Chen Chen (6544)
Fen Li (337013)
Bo-kui Xiao (498024)

December 2013

Key proteins related to cycle regulation were detected by WB. As I3C concentration increased, cyc...

Restriction point and metabolic checkpoint arrest lead to differential patterns of cell cycle regulator expression and phosphorylation.

Mahesh Saqcena (447633)
Deepak Menon (447634)
Deven Patel (376312)
Suman Mukhopadhyay (190415)
Victor Chow (447635)
David A. Foster (447636)

August 2013

(A) Cells were plated at 30% confluence in 10-cm plates in DMEM containing 10% FBS. After ...

Immunoblotting analysis of protein levels.

Carolina Vizcaíno (246726)
Luz-Elena Núñez (169843)
Francisco Morís (169876)
José Portugal (30112)

August 2014

(A) Western blots showing changes in protein levels in A2780 cells treated with 80 nM DIG-MSK for...

Western blot analysis of cell cycle related proteins of MCF-7 cells treated with 300 µg/ml PRME.

Abstract

Extracted data

Western blot analysis of cell cycle related proteins of MCF-7 cells treated with 300 µg/ml PRME.

Abstract

Extracted data

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