Changes in monocyte phenotype upon entry to the wound.

<p>Gated F4/80⁺Ly6C^hi blood monocytes from naïve and day 1-wounded C57BL/6J mice were compared to wound monocytes from day 1-wounded mice by flow cytometry analysis. (A) Proportion of TNF-α⁺ cells and representative FACS plots, (B) proportion of CD14⁺ cells and representative histograms, (C) FSC median fluorescence intensity (MFI) and (D) SSC MFI with representative histograms. Data shown are the mean ± SD, n = 3 mice per group for (A) and (B); n = 2–3 mice per group for (C) and (D). Data are representative of 2–3 independent experiments.