The skin wound induced a rapid and transient wave of monocyte recruitment.

<p>(<b>a</b>) Representative TPLSM picture of the wound deep skin layer from MacBlue×CX3CR1^gfp/+ and MacBlue×CX3CR1^gfp/+ CCR2^−/− mice at several time points after wounding. The SHG signal is in blue, the ECFP signal in cyan, and the GFP signal in green. (<b>b</b>) Quantification of ECFP⁺ cell density from TPLSM pictures taken 15 to 360 min post-wounding in MacBlue×CX3CR1^gfp/+ and MacBlue×CX3CR1^gfp/+ CCR2^−/− mice. Each dot represents a mean number of ECFP cells calculated from 30 to 50 different fields (100×100 µm) from 3–8 mice. Time points were compared using one way ANOVA followed by Bonferroni adjustment. (<b>c</b>) Representative flow cytometry dot plots of Ly6C and Ly6G expression by ECFP⁺ blood myeloid cells (gated on CD11b⁺, NK1.1⁻) from MacBlue×CX3CR1^gfp/+ mice before and 4 hours after wounding. (<b>d</b>) Quantification of ECFP⁺ Ly6C^high inflammatory and Ly6C^low resident monocytes in the blood of MacBlue×CX3CR1^gfp/+ and MacBlue×CX3CR1^gfp/+ CCR2^−/− mice before and 4 hours after injury (n = 4–7 from 3 independent experiments). Inf Mo: Inflammatory monocytes; Resident Mo: Resident monocytes. *p<0.05; **: p<0.01; ***: p<0.001.