Chemokine receptor expression on circulating and wound monocytes/macrophages.

<p>(A) Blood cells were isolated from naïve, day 1 and day 7-wounded C57BL/6J mice. Gated F4/80⁺SSC^low blood monocytes were examined for Ly6C expression in naïve and day 1-wounded mice. Mean frequencies (±SD) are indicated above each gate. The mean number ± SD of F4/80⁺Ly6C^hi and Ly6C^low blood monocytes is also shown. (B) Expression of CX₃CR1 was compared on circulating and wound F4/80⁺ monocyte/macrophage subsets at 1 and 14 days after wounding by flow cytometry using CX₃CR1-GFP transgenic mice. (C) C57BL/6J or CX₃CR1-GFP transgenic mice were wounded for 1 or 14 days. Gated F4/80⁺Ly6C^hi and Ly6C^low wound monocytes/macrophages were examined by flow cytometry for CCR2 and CX₃CR1 expression. Average MFI ± SD (geometric mean) is indicated in each plot. N = 3–5 mice per group. Data are representative of at least 3 independent experiments.