Monocytes infiltrated the wound bed concomitantly with neutrophils.

<p>(<b>a</b>): Time lapse picture series representing concomitant invasion of the wound bed by monocytes (black arrows) and neutrophils (red arrows). The SHG signal is in blue, the ECFP signal in cyan, and the PE signal in red. Representative track paths are represented by white dashed lines. (<b>b</b>) Representative TPLSM pictures of the wound deep skin layer from MacBlue×CX3CR1^gfp/+ mice at several time points after wounding. Mice were injected intravenously with 10 µg of Ly6G-PE antibody before wounding. The SHG/ECFP signal is in blue, and the PE signal in red. (<b>c</b>) The graph shows the quantification of Ly6G⁺ and ECFP⁺ cell density from TPLSM pictures taken 15 to 360 min post-wounding in MacBlue×CX3CR1^gfp/+ mice. Each dot represents a mean of number of ECFP⁺ cells calculated from 30 to 50 different fields (100×100 µm) from 3 to 8 different mice. Time points were compared using one way ANOVA followed by Bonferroni adjustment. *p<0.05; **: p<0.01.