UNC93B1 is localized in the ER and in the lysosomes.

<p>(A–D) HEK293T cells were transfected with a UNC93B1-mCherry-Myc (cyan). (A) ER was dyed with ER-Tracker Blue-White DPX. (B) Lysosomes were marked with LysoTracker Green DND-26. (C) Endosomes were stained with Transferrin AlexaFluor 633 conjugate. (D) Plasma membrane was dyed with CTB-Alexa 647. All dyes are shown in magenta. White arrows indicate colocalization. (E) HEK293T cells were transfected with a UNC93B1-mCherry-Myc (magenta) and TLR3-mCer (cyan). Plasma membrane was dyed with CTB-Alexa 647 (red). Membrane localization was evaluated from plots of normalized fluorescence intensities of UNC93B1-mCherry-Myc and TLR3-mCer and plasma membrane (PM) within 3 μm line profiles (n = 9). Three representative lines are marked on merged images. Images are selected from three independent experiments. Scale bars, 10 μm.