Localization of chimeric receptors with exchanged TM segments of TLR3 and TLR9.

<p>HEK293T cells were transiently transfected TLR3-9-3-mCer (A and B - cyan), TLR9-3-9-YFP (C and D - yellow), and with UNC93B1. Plasma membrane markers SynaptoRed and CTB-Alexa 555 are shown in magenta. (A) Localization of TLR3-9-3-mCer on plasma membrane in cells overexpressing UNC93B1. (B) Intracellular localization of TLR3-9-3-mCer in HEK293T without overexpression of UNC93B1. (C) Intracellular localization of TLR9-3-9-YFP in cells overexpressing UNC93B1. (D) Intracellular localization of TLR9-3-9-YFP in HEK293T without overexpression of UNC93B1. (A-D) Data are representative of three experiments. TLR membrane localization was evaluated from plots (bottom) of normalized fluorescence intensities of TLR and plasma membrane (PM) within 3 μm line profiles (n = 9). Three representative lines are marked on merged images. Images are selected from three independent experiments. Scale bars, 10 μm.