Co-localization of transfected cycD2SV with markers of ER stress and autophagy.

<p>HEK293 cells co-transfected with cycD2SVmyc and TCRα-GFP (A–C), cycD2SVmyc and ubiquitin (D–F), cycD2SVmyc and YFP-intersectin (J–L) and cycD2SV-EGFP and mcherry-p62 (M–O) were processed for myc (A, D, G), HA (E) immunostaining and nuclear stain (C, F, I, L). Co-localization of singly transected cycD2SV aggregates in γ-tubulin positive MTOC (G–I). HEK293 cells transfected with cycD2SVmyc (G–I) processed for myc (G), γ-tubulin (H) immunostaining and nuclear stain (E). Scale bar is 20 µm.