Cytosolic PanK1β partially associates with clathrin-coated and recycling endosomes.

<p>HEK293 cells were transfected with expression plasmids encoding mPanK1β fused to ZsGreen1 (green, a, c, d, f, g, i, j, l, m, o), together with different plasmids encoding the fluorescent protein mCherry fused to distinct markers for different subcellular vesicles: human clathrin LCB (magenta, b, c), human Rab5 (magenta, e, f), human Rab11 (magenta, h, i), firefly luciferase peroxisomal targeting signal (magenta, k, l) and rat Lamp1 (magenta, n, o). Rab5 designates early endosomes, Rab11 designates recycling endosomes and Lamp1 designates lysosomes. Cells were visualized using live-cell confocal imaging. Merged images show co-localization as indicated by white pixels resulting from both magenta and green pseudocolored contributions. Insets in merged images show details at higher magnification. Results are representative of 2 or more independent experiments. Scale bar, 10 µm.