Internalisation of OlyA-mCherry in MDCK cells.

<p>(<b>A–H</b>) Representative fluorescent images of MDCK cells (blue, DAPI; red, OlyA-mCherry) showing immunolabelling of respective membrane domains (green, as indicated) and labelling of OlyA-mCherry (1 μM). After 5 min, OlyA-mCherry shows “weak/moderate” co-localisation with clathrin on the plasma membrane (<b>A</b>), and “moderate” with caveolin-1 (<b>B</b>) and EEA-1 (<b>C</b>). After 30 min, OlyA-mCherry shows “strong” co-localisation with caveolin-1 (<b>D</b>) and “weak/moderate” with cathepsin L (<b>E</b>). After 90 min, OlyA-mCherry shows “moderate” co-localisation with the GM130 (<b>F</b>) and P230 (<b>H</b>) markers of the Golgi apparatus, and “weak/moderate” for giantin (<b>G</b>). (<b>I</b>) Quantitative analysis of the co-localisation between OlyA-mCherry and the other membrane markers. Co-localisation in (F–H) are restricted to individual spots. Scale bars: 20 μm.