Localisation of proteins in secretory vesicles.

<p><i>A</i>. localisation of TGFβig-h3 (green) and MMP2, MIF or SPACR (red) in MSCs revealed by immunocytochemistry. In each case there are images for the red channel, green channel, a marge of these two images, and finally a merged image at higher magnification. There are clear examples of TGFβig-h3 and either MIF or MMP-2 in similar vesicular populations (yellow or orange), while TGFβig-h3 and SPARC are localised to distinct vesicular structures (red or green vesicular structures). Nuclei stained blue with DAPI. Scale bars 5 or 10 μm. <i>B</i>. TEM identifies dense core secretory vesicles in MSCs. representative photomicrograph taken at a magnification of 60,000x show dense core secretory vesicles (closed arrows) in MSCs the Golgi apparatus is clearly visible (open arrows). Scale bar 500nm.