Structural alterations do not affect translocation of chimeric receptors from ER to endosomes and lysosomes.

<p>HEK293T cells were transiently transfected with TLR3-mCer, TLR9-YFP, TLR3-9-3-mCer, TLR9-3-9-YFP, TLR3-3-9-YFP or TLR9-9-3-YFP. ER was dyed with ER-Tracker Red, lysosomes were marked with LysoTracker Red DND-99. To stain endosomes, cells were cotransfected with EEA1-Tomato. All dyes are shown in magenta. White arrows indicate colocalization. Images are selected from three independent experiments. Scale bars, 10 μm.