Mobilization of gTLR9A and gTLR9B upon stimulation with CpG ODN.

<p>(A) In non-stimulated cells (0 min), gTLR9 is primarily localized perinuclearly in a diffused interconnected web pattern resembling that of the ER. Upon stimulation with ODN, TLR9A gradually formed large aggregates at the location of the presumed endosome. (B) gTLR9B exhibited a pattern similar to that of gTLR9A. These results indicate that both gTLR9A and 9B can be mobilized from the perinuclear area to the endosome. The exact location of the endosome was verified as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126388#pone.0126388.g006" target="_blank">Fig 6</a>. Results are presented as the observation of at least 100 transfected cells from one representative experiment out of two.