Intestinal injury in wild-type mice is not associated with a selective reduction in γδ IEL.

<p>A) Flow cytometry of small intestinal intraepithelial cells isolated from 2 weeks old dam fed wild-type (WT control, n = 2) or wild-type mice subjected to experimental gut injury as described (WT⁺PAF/LPS, n = 2). C57BL/6J mice stained for CD103, CD3, CD8α, TCRγδ and TCRβ as described above. Intraepithelial cells were pregated on CD103⁺, CD3⁺ to depict IEL and then further gated on TCRγδ and TCRβ as shown. B) Percent (mean ±SE) γδ IEL (defined as percent of total IEL that were TCRγδ⁺, TCRβ^- IEL). Data are representative of 3 independent experiments (NS indicates no statistical difference between groups).