GPR18 is not required for steady state intestinal IELs.

<p>(A) H&E sections of small intestine from <i>Gpr18</i>^<i>+/+</i> and <i>Gpr18</i>^<i>-/-</i> mice. White arrows depict examples of IELs. Insets show relevant portions at higher magnification. Sections were obtained from duodenum of small intestine, and are representative of 2 mice. (B) Numerical analysis of small intestine IELs from <i>Gpr18</i>^<i>+/+</i>, <i>Gpr18</i>^<i>+/-</i>, and <i>Gpr18</i>^<i>-/-</i> mice. Frequencies of TCRγδ (B) and TCRαβ (C) IELs were determined by flow cytometry, and IEL absolute numbers were calculated using the total recovered cell number from the small intestine. Mean values ± SEM are shown. Each symbol represents one mouse. n.s., not significant using 1-way ANOVA.