γδ IEL are the predominant IEL subtype in the immature murine small intestine.

<p>A) Flow cytometry of distal small intestinal intraepithelial cells from 1 week old (1 wk, n = 4), 2 weeks old (2 wk, n = 3), 3 weeks old (3 wk, n = 2) and adult (n = 1) C57BL/6J mice stained for CD3, CD8α, CD103, TCRγδ and TCRβ as described. Intraepithelial cells were pregated on CD103⁺, CD3⁺ to depict IEL and then further gated on TCRγδ and TCRβ as shown. B) Percent (mean ±SE) γδ IEL (defined as percent of total IEL that were TCRγδ⁺, TCRβ⁻ IEL) in the distal small intestines of 1 wk, 2 wk, 3 wk, and adult mice. Data are representative of 3 independent experiments (*p<0.05 when compared to 1 wk samples).