The number of CD4 TCR-β cells in the spleens, MLNs, and large intestines of recipient mice was assessed at the indicated times

(A) 1 wk after transfer, (squares) and (circles) mice, four in each group, were analyzed by flow cytometry for T cells in the spleens, large IELs, and MLNs. (B) Shows same as in A, but including LPLs and analyzed 2 wk after transfer. Each symbol represents an individual mouse with a total of 7–10 mice per group. *, P < 0.05; **, P < 0.005. (C–E) Real-time PCR performed on tissue samples isolated from the large intestines of individual (filled bars) or (open bars) recipients 2 wk after transfer. Error bars represent SD of triplicate measurements. (F) LPLs isolated from transferred (open bars) and (filled bars) recipients at 2 wk were stimulated ex vivo with PMA and ionomycin. After 48 h, cytokines in the supernatant were measured by ELISA.<p><b>Copyright information:</b></p><p>Taken from "A crucial role for HVEM and BTLA in preventing intestinal inflammation"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1463-1476.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413041.</p><p>