GFP⁺Ly6C^low monocytes exhibit patrolling behavior, while GFP⁺Ly6C^high cells transit rapidly through the sinusoids.

<p>(<b>A</b>) High magnification snapshot showing tracks of GFP+ crawling cells (white) and GFP+ cells that move rapidly through the sinusoids (yellow) in a steady state uninfected liver. Scale bar = 50 µm. (<b>B</b>) Representative tracks of crawling monocytes in steady state uninfected livers. (<b>C and E</b>) Intravital confocal microscopy of an uninfected <i>Cx₃cr1^gfp/+</i> mouse showing GFP+ (green) monocytes that are either Ly6C^high (<b>C</b>) or Ly6C^low (<b>E</b>) after intravenous anti-Ly6C Ab staining (red). (<b>D and F</b>) Time-lapse images from an uninfected <i>Cx₃cr1^gfp/+</i> mouse injected with anti-Ly6C Ab showing two GFP+ Ly6C^high monocytes (<b>D</b>) and two GFP+ Ly6C^low monocytes migrating through the sinusoids (<b>F</b>). GFP is shown in green, anti-Ly6C in red, and nuclei in blue. Snapshots were taken in single z planes. Scale bars = 20 µm. Tracks are shown in white.