Migration patterns of GFP-positive and GFP-negative cells.

<p>A: representative <i>in vivo</i> microscopy images show transmigration of GFP-positive (GFP-image) and GFP-negative cells (RLOT-image) at 60 min after microinjection of MCP-1 in Cx3CR1^gfp/gfp mice (objective magnification 20×; asterisk shows the site of microinjection). B–E: The motility parameters, curve-line migration velocity (B), straight-line migration velocity (C), and directionality (D) of interstitially migrating, GFP-positive (monocytes, gray bars) and GFP-negative cells (neutrophils, black bars) were analyzed at 60, 70, and 80 min after microinjection of MCP-1 for 5 min, respectively; mean±SEM; *p<0.05 vs. GFP-negative cells; # p<0.05 vs. 60–65 min; n = 5. E: polarization of GFP-negative cells (black bars) and GFP-positive cells (gray bars) analyzed at 70 min after microinjection of MCP-1; mean±SEM, n = 15.