Wound-infiltrating monocytes migrated actively and then stopped within the matrix.

<p>(<b>a</b>) Representative TPLSM picture of monocyte migratory behavior 210 min after injury of MacBlue×CX3CR1^gfp/+ mice. Track pathways of ECFP⁺ cells (cyan) are indicated. Quantification of ECFP⁺-cell mean velocity (<b>b</b>) and arrest coefficient (<b>c</b>) at indicated time range after injury. (<b>d</b>) Graphs represent cell frequency distribution as a function of their mean velocity at indicated time range after injury. The percentages of cells with a mean velocity higher than 12 µm/min are indicated. (<b>e</b>) Quantification of track straightness of ECFP⁺ cells at the indicated time range after injury. (<b>f</b>) Representation of the ECFP⁺-cell mean displacement as a function of the square root of time at 210 min after injury (all quantifications represent a pool of 27 to 781 cells from 3 to 6 different videos performed on 2 to 5 different mice). *p<0.05; ***p<0.001.