CX₃CR1-GFP⁺ monocytes accumulate in the liver when <i>S. mansoni</i> eggs appear without proliferation.

<p>(<b>A–C</b>) Representative flow cytometry plots and graphs display the proportion of GFP⁺ Ly6C^high and Ly6C^low monocytes from CX₃CR1^GFP/+ mice gated on live, single, lineage (CD3, B220, DX5 and Siglec F) negative, SSC^low cells. (<b>D–E</b>) qRT-PCR analysis of CCR2 and eGFP expression in the liver. (<b>F</b>) Representative contour plots show <i>in situ</i> EdU labeling of liver leukocytes gated on live, single, lineage negative, CD11b⁺ cells. Results are representative of two experiments that included n = 3–4 mice/group. Statistical significance was determined by One-way ANOVA with Dunnett's test. * = p<0.05, ** = p<0.001, *** = p<0.0001.