Ly6C^- monocytes induce differentiation of Ly6C⁺ monocytes into macrophages in <i>T</i>. <i>congolense</i>-infected WT mice.

<p><b>(A)</b> Liver MHC-II^{- to lo} Ly6C^- monocytes (Ly6C^- Mo) purified from CX₃CR1-GFP^+/- mice at day 21 pi were transferred in CD45.1 WT mice at day 4 and day 6 pi. Control mice received HBSS. At day 7 pi, percentages of Ly6C⁺ monocytes (Ly6C⁺ Mo), Ly6C^- monocytes (Ly6C^- Mo) and macrophages (MF) within liver non-parenchymal cells were determined in recipient mice. Data are shown as mean + SD of 3 individual mice of one representative out of three independent experiments. *p<0.05 compared to control mice. <b>(B)</b> GFP⁺ Ly6C⁺ monocytes purified from CD45.2 LysM-GFP mice at day 7 pi were transferred in CD45.1 WT mice at day 12 pi with or without MHC-II^{-to lo} Ly6C^- monocytes gated as described in S5A Fig in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004873#ppat.1004873.s001" target="_blank">S1 Text</a> and purified from CD45.2 WT mice at day 21 pi. After 48 h, liver CD45.2⁺ GFP⁺ cells in recipient mice were analyzed for CD11b and Ly6C expression. MHC-II expression was then investigated in Ly6C^- CD11b⁺ cells. FACS profiles are representative of 1 out of 9 mice tested in three independent experiments. Percentages of cells in indicated gates are shown as mean ± SD of 3 individual mice of one representative out of three independent experiments. <b>(C)</b> TNF and <b>(D)</b> IL-10 concentration in supernatants of liver non-parenchymal cell cultures from recipient mice. <b>(E)</b> ALT levels in blood serum from recipient mice. Data are shown as mean + SD of 3 individual mice of one representative out of three independent experiments. *p<0.05 compared to recipient mice receiving only Ly6C⁺ monocytes.