Evaluation of NP specific IgG and IgG subclasses in serum.

<p>Anti-NP IgG titers were determined by ELISA on day 60. BALB/c mice (n = 5) were vaccinated with two doses of rNP (10 µg/dose) alone or co-administered with c-di-AMP (10 µg/dose) by intranasal route, whereas control animals received PBS, at an interval of 3 weeks. (A) Anti-NP IgG titer. (B) Anti-NP IgG subclass titers. The results are expressed as mean end point titers. The S.E.M. is indicated by vertical lines. Differences were statistically significant at p<0.01 (**) or p<0.05 (*) with respect to values obtained in mice receiving the antigen alone.