Immunization with IDLV-NP induces NP-specific IgG responses that are increased upon boosting.

<p>Mice were inoculated 2 times i.n. or i.m. with IDLV-NP, 4 weeks apart. Mice that received either PBS or IDLV-GFP 1 or 2 times i.n. served as a negative control. Serum was collected from each mouse prior to the first immunization, prior to the second immunization (1X), and 4 weeks after the second immunization (2X). NP-specific IgG in serially-diluted serum samples was measured by indirect ELISA assay and is expressed as endpoint titers, defined using the pre-immunization sera as a baseline. Each dot in the panel represents one mouse, and bold lines indicate the mean. The lower limit of detection is indicated by the dotted line. The Kruskal-Wallis test was used to compare antibody titers between the groups (overall p-value of <0.0001). P-values from pairwise group comparisons based on the ANOVA test are shown.