Antibody titers in sera of mice following priming vaginal immunization with 20 nm NP-Ova and s.c. boosting immunization with Ova in CFA.

<p>Mice were immunized vaginally with 20 nm NP-Ova or PBS (control), then s.c. boosted with 300 µg Ova with CFA at day 28 after priming immunization (arrows). (A) Ova-specific IgG1 and (B) IgG2c antibody titers are expressed as log₁₀ titer values, with the titer being the highest dilution that yielded an OD₄₀₅ absorbance value two times that of the negative control. Values represent the mean ± SD of samples collected from 14 mice per group (3 separate experiments). Group means were separated using Student's t-test and declared significantly different at a P<0.05. Asterisks indicate significant differences between treatment means.