Antibody titers in fecal extracts of mice vaginally primed with 20 nm NP-Ova (or PBS, control) and s.c. boosted with 300 µg Ova with CFA at day 28 after priming immunization (arrows).

<p>Fecal pellets were collected each week from individual mice and fecal extracts were assayed by ELISA. (A) Ova-specific sIgA titers in fecal extracts. (B) Ova-specific IgG1 titers in fecal extracts. Antibody titers are expressed as log₁₀ titer values, with the titer being the highest dilution that yielded an OD₄₀₅ absorbance value two times that of the negative control. Values represent the mean ± SD of samples collected from 14 mice per group (3 separate experiments). Group means were separated using Student's t-test and declared significantly different at a P<0.05. Asterisks indicate significant differences between treatment means.