Anti-OVA IgG level in sera of immunized mice.

<p>Immunization by intra-rectal route with ovalbumin as antigen (OVA), with cholera toxin (CT) (n = 14), <i>Salmonella</i> Typhimurium flagellin (FLA-ST) (n = 14), <i>C</i>. <i>difficile</i> flagellin (FliC) (n = 14) respectively as adjuvant and PBS administration for the control group (n = 14). Immunization by intra-peritoneal route with OVA as antigen and FliC as adjuvant (FliC IP) (n = 8). D0: before immunization, D15: first immunization, D30: first boost, D45: second boost. *: statistically significant difference *: 0.01<<i>p</i> < 0.05; **: 0.001<<i>p</i><0.01; ***: <i>p</i><0.001 (student t-test). Dotted-line corresponds to the cut-off value.