NiV VLP-induced immune response in Balb/c mice.

<p>NiV-specific antibody levels of serum samples from mice immunized subcutaneously three times were measured by IFA and by Bio-Plex microsphere methods. Neutralizing antibody response was evaluated by PRNT₅₀. The experiments were done in duplicate. <b>A</b>: For evaluation by IFA, sera from each treatment group were pooled for analysis. The results show serocoversion for each of the four treatment groups. In general, the titers increased progressively with time and with the VLP dose although by day 35, similar titers were seen with the three higher VLP doses. <b>B</b>: Shows neutralizing antibody titers (PRNT₅₀) in sera from each mouse collected on the stated days. Neutralizing antibodies were seen starting on day 28 after primary inoculation. The response was again clearly dose dependent; all mice in the two highest treatment groups (C and D) showed neutralizing response by day 35. Such response was seen in 3 of 5 and 1 of 5 mice in the two lower (B and A respectively) treatment groups.