Viral clearance and antibody titre induced by immunisation with the low dose combination vaccine and its individual components.

<p>Mice were immunised once by the i.n. (squares), s.c. (triangles) or i.m. (circles) routes with 10 nmol lipopeptide (LP), 0.3 µg of HA as split virus (SV) or a combination of both components as indicated (+) or infected with 10^4.5 pfu of virus (Inf). Control mice received PBS. One month post-immunisation, mice were challenged by the i.n. route with 10^4.5 pfu of virus, lungs were removed 5 days later and pulmonary viral titre determined by plaque formation (A). Mice were bled prior to challenge, and the virus-specific antibody response in sera measured by ELISA (B). Symbols represent the titres for individual mice, and the line indicates the geometric mean for the group (n = 5). The numbers above the data sets refer to the average percentage decrease in viral load compared to PBS control mice.