Induction of anti-rPspA IgG serum antibodies.

<p>Mice were immunized intranasally with two doses of the indicated formulations and anti-rPspA1(A), anti-rPspA2 (B), anti-rPspA3 (C) and anti-rPspA4 (D) IgG in serum samples were detected by ELISA. * indicates statistical difference with saline (One-way ANOVA, Tukey’s Multicomparison Test—*** P≤0.001). Results are representative of two independent experiments.