Anti-PmpC antibody serum levels.

<p>Levels of (A) anti-N-PmpC IgA and (B) anti-N-PmpC IgG and (C) ratio of the levels of N-PmpC-specific IgG1 and IgG2a antibodies in the serum of BALB/c mice immunized via the conjunctiva and age-matched normal controls (nc). All serum samples were collected two weeks after completion of the indicated immunization protocol and were assayed by ELISA. Results for each individual serum sample are presented. The levels of N-PmpC-specific IgG1 and IgG2a were judged according to the A_492/620 values recorded for individual serum samples. The statistical significance of the observed differences was evaluated using Kruskal -Wallis test followed by Dunn's multiple comparisons test to compare between groups (<i>P</i> < 0.05*, <i>P</i><0.005**). Compared groups are indicated by two-head arrow.