NP protein delivered with alum primes a specific T and B cell immune response.

<p>B6 mice were immunized i.m. in both hind-legs with a total of 10 µg of NP protein delivered with or without 100 µg of alum. The percentages (A, B) or numbers (C, D) of D^b/NP_366–74 CD8 or IA^b/NP_311–25 CD4 T cells were examined in the spleen or the two popliteal lymph nodes (DLN) 9 days later. Cells are gated on CD8+dump negative live cells (A) or CD4+dump negative live cells (B) with the number in the plot indicating the percentage of CD8 or CD4 cells that are CD44^hi tetramer+ as indicated by the gate. In C and D each point represents a mouse and the line shows the mean of the group. The X axis is set at the level of detection, determined by staining cells from naïve animals with the MHC tetramers. Serum from these animals was used to examine the level of NP specific IgG1 antibody (E), with each line representing one animal. These data are combined from 2 independent experiments with 3 mice per group.