Ag85B protein stimulation in spleen cells of NP-mice induces expression of 4-1BB on both CD4⁺ and CD8⁺ T cells.

<p>Pooled spleen cells of NP-mice (panel A) or P-mice (panel B) were re-stimulated with Ag85B protein (5 µg/ml). After 3 or 6 days of cultures, cells were labelled with the following mAb: CD4 PE-Cy7, CD8 PE-Cy7, CD3 FITC and 4-1BB PE. Cell surface molecule expression was analyzed by flow cytometry as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011019#s4" target="_blank">Materials and Methods</a>. Within each panel, the percentage indicates the number of cells expressing the 4-1BB receptor. Plots, gated on CD4⁺ or CD8⁺ T cells in the lymphocyte-gate, are representative of one experiments out of 3.