Pre-treatment of EPO decreases caspase-3 activity in H/R induced H9C2 cells.

<p>The effect of EPO on caspase-3 activity was determined using caspase-3 colorimetric assay. H9C2 cells were subjected to H/R with or without pre-treatment with 20 U/ml EPO for 24 hrs and incubated with 1 µM of Wortmannin 30 mins before EPO treatment. EPO decrease caspase-3 activity after H/R. H9C2 cells pretreated with wortmannin showed increase in caspase-3 activity. Data are presented as means ± SEM of the ratios from three independent experiments * denotes p<0.001 for analyses compared to H/R.