Pre-treatment of EPO increases intracellular p-Akt in H/R induced H9C2 cells.

<p>The effect of EPO on p-Akt was determined. H9C2 cells (A) were subjected to 8 hrs hypoxia and 30 mins of reperfusion with or without pre-treatment with 20 U/ml EPO and 1 µM Wortmannin before 30 mins for 24 hrs and stained with p-AKT antibody. EPO increase p-Akt. (B) Represents the quantification green florescence, which indicates the increase in levels of p-Akt. Data are presented as means ± SEM of the ratios from three independent experiments * denotes p<0.001 for analyses compared to H/R.