Effect of ephrin-A1-Fc on expression and phosphorylation of eNOS and Akt in HUVECs. HUVECs were exposed to ephrin-A1-Fc (1 µg/ml) for 0 h, 8 h, 24 h.

<p>Immunofluorescence (A), Real-time PCR (B) and Western blot analysis (C) demonstrated that ephrin-A1-Fc had no effect on eNOS expression of HUVECs (#, <i>P</i>>0.05, n = 3). D: Western blots demonstrating that P-eNOS_Ser1177 and P-Akt_Ser473 were up-regulated under ephrin-A1-Fc stimulation in a time-dependent manner. E: Quantity analysis of P-eNOS_Ser1177. F: Quantity analysis of P-Akt_Ser473. (*, <i>P</i><0.05, n = 4).