Pre-treatment of EPO maintains mitochondrial membrane potential in H/R induced H9C2 cells.

<p>The effect of EPO on Membrane potential was determined by colocalization studies using Rhodamine-123 and DCFH-DA. H9C2 cells were subjected to H/R with or without pre-treatment with 20 U/ml EPO for 24 hrs. H9C2 cells pretreated with EPO and control cells showed Rhodamine-123 fluorescence only in the perinuclear region about 58% and 52% colocalzation respectively whereas H9C2 cells subjected to H/R showed Rhodamine-123 fluorescence both in perinuclear region and cytosol about 94.7% co localization in Figure 5 A. Data are presented as means ± SEM of the ratios from three independent experiments * denotes p<0.001 for analyses compared to H/R.