<p>(<b>A, B</b>) HBMEC (<b>A</b>) and Caco-2 cells (<b>B</b>) were incubated with different doses of TA50 or 8033 OMVs (20 µg or 10 µg of OMV protein containing 100 ng or 50 ng of EHEC-Hly, respectively) or with 20 µg of EHEC-Hly-negative OMVs (TA51 or 8033c) for the times indicated or remained untreated. Cells were lysed, the lysates were incubated with colorimetric substrates of caspase-9 (Ac-LEHD-pNA) or caspase-3 (Ac-DEVD-pNA) and the color intensity, which is proportional to the level of caspase enzymatic activity, was measured spectrophotometrically. The activity of each caspase in OMV-treated cells was expressed as a fold-increase of that in untreated cells (defined as 1). Specific inhibitors of caspase-9 (z-LEHD-fmk), caspase-3 (z-D...
<p>(A). Lysates were prepared from Cal27 cells treated or untreated with BME for 72 h, and subjected...
<p>(A) Activated apoptosis in proliferating B cells treated with DMSO (top panels) or Chk2i (bottom ...
<p>A) Caspase-3 activation. Cells were grown on a glass coverslips and treated or not with 70 µM hyd...
<p>(<b>A, B</b>) HBMEC and Caco-2 cells were incubated with EHEC-Hly-containing (TA50 or 8033) or EH...
<p>(A-H) Lysosomes (Lyso) and mitochondria (Mito) were isolated from HBMEC and Caco-2 cells, which h...
<p>The effect of EPO on caspase-3 activity was determined using caspase-3 colorimetric assay. H9C2 c...
<p>(<b>A</b>) Time course evaluation of caspase 3, 8, and 9 activation and PARP cleavage in EV71-inf...
<p>(<b>A, B</b>) HBMEC (<b>A</b>) and Caco-2 cells (<b>B</b>) were incubated for the times indicated...
<p>Caspase activity assays were performed as described in Materials and Methods. Peptides containing...
(A-B) Immunoblots for EthA in different fractions of E. tarda culture at 10 h post-inoculation (A) a...
<p>(A) U937 cells were treated for 14 and 24 h with 1.4 µg/ml HF or left untreated. Caspase-8, -9 an...
<p>Infection with purified HCP, wild-type O157:H7 and the <i>hcpA</i> mutant showing percentages of ...
<p>(<b>A</b>) HBMEC and Caco-2 cells either pretreated with bafilomycin A1 (BafA1+) (100 nM, 1 h) or...
<p><b>(</b>A) Representative image (top) and quantitative analysis (bottom) of Western blot of cleav...
(A) 116 hours after transduction, sh control and ATP9A knock-down cells were incubated with lactadhe...
<p>(A). Lysates were prepared from Cal27 cells treated or untreated with BME for 72 h, and subjected...
<p>(A) Activated apoptosis in proliferating B cells treated with DMSO (top panels) or Chk2i (bottom ...
<p>A) Caspase-3 activation. Cells were grown on a glass coverslips and treated or not with 70 µM hyd...
<p>(<b>A, B</b>) HBMEC and Caco-2 cells were incubated with EHEC-Hly-containing (TA50 or 8033) or EH...
<p>(A-H) Lysosomes (Lyso) and mitochondria (Mito) were isolated from HBMEC and Caco-2 cells, which h...
<p>The effect of EPO on caspase-3 activity was determined using caspase-3 colorimetric assay. H9C2 c...
<p>(<b>A</b>) Time course evaluation of caspase 3, 8, and 9 activation and PARP cleavage in EV71-inf...
<p>(<b>A, B</b>) HBMEC (<b>A</b>) and Caco-2 cells (<b>B</b>) were incubated for the times indicated...
<p>Caspase activity assays were performed as described in Materials and Methods. Peptides containing...
(A-B) Immunoblots for EthA in different fractions of E. tarda culture at 10 h post-inoculation (A) a...
<p>(A) U937 cells were treated for 14 and 24 h with 1.4 µg/ml HF or left untreated. Caspase-8, -9 an...
<p>Infection with purified HCP, wild-type O157:H7 and the <i>hcpA</i> mutant showing percentages of ...
<p>(<b>A</b>) HBMEC and Caco-2 cells either pretreated with bafilomycin A1 (BafA1+) (100 nM, 1 h) or...
<p><b>(</b>A) Representative image (top) and quantitative analysis (bottom) of Western blot of cleav...
(A) 116 hours after transduction, sh control and ATP9A knock-down cells were incubated with lactadhe...
<p>(A). Lysates were prepared from Cal27 cells treated or untreated with BME for 72 h, and subjected...
<p>(A) Activated apoptosis in proliferating B cells treated with DMSO (top panels) or Chk2i (bottom ...
<p>A) Caspase-3 activation. Cells were grown on a glass coverslips and treated or not with 70 µM hyd...