Caspase-3 activity was increased following the treatments.

<p>Sodium azide/2-DG, increased temperature or combination of sodium azide/2-DG plus increased temperature resulted in increased caspase-3 activity. Cells were pre-incubated with DMSO or caspase-3 inhibitor (z-DEVD-FMK) for 2 hours, and then cells were subjected to the indicated treatments for 1 hour. * denotes significant difference from the 37°C DMSO cell (<i>p</i><0.01); † denotes significant difference from the 37°C plus sodium azide/2-DG and 41°C alone cells (<i>p</i><0.05); ‡ (<i>p</i><0.05) and § (<i>p</i><0.01) denote significant difference from the DMSO groups, respectively. n = 3.