All three LNPs facilitated GAPDH mRNA silencing in Caco-2 cells.

<p><b>A)</b> LNPs 306O₁₃ and 303O₁₃ mediated ~90% silencing at an siRNA dose of 100 nM while control LNPs containing off-targeted siRNA did not induce statistically significant changes in gene expression. ** p < 0.001 as determined by an unpaired student’s t-test to the untreated control. <b>B)</b> The 306O₁₃ LNPs had the most potent dose-dependent GAPDH gene silencing in Caco-2 cells. The LNP control consisted of 100 nM siGFP 306O₁₃ LNPs. Error bars represent s.d. (n = 3).