Knockdown of GAS5 suppresses the effects of serum withdrawal on the expression of glucocorticoid-responsive genes.

<p>HEK293 cells were treated with a control siRNA or with a siRNA targeting GAS5, and cultured either with or without serum for 48 h. (A, B) The relative expression levels of the indicated RNAs in cells with serum (black bar) or without serum (grey bar) relative to control (cells cultured at 0 h) were determined by RT-qPCR. The levels of GAPDH and ACTB were used for normalization. Values represent mean±SD obtained from three independent experiments (*<i>P</i><0.05, **<i>P</i><0.01, Student's t test).