Reduced Zip7 expression reduces the expression of Glut4 and decreases glycogen synthesis.
- Stephen A. Myers (483447)
- Alex Nield (483448)
- Guat-Siew Chew (483449)
- Mark A. Myers (483450)
Publication date
November 2013
DOIAbstract
<p>A). Relative expression of <i>Glut4</i> mRNA to <i>Eef2</i> in the scramble control and siRNA-<i>Zip7</i> C2C12 cells. B). Western blot for immunoreactive Glut4 and Gapdh in protein lysates from the scramble control and the siRNA-<i>Zip7</i> C2C12 cells. C). Assay for glycogen synthesis in scramble and siRNA-<i>Zip7</i> cells treated with 10 nM insulin for 1 hour. D). Error bars indicated the ± SD from three independent biological samples for <i>Glut4</i> mRNA, and six independent transient transfections of the siRNA-<i>Zip7</i> for the glycogen synthesis. **<i>P</i><0.01, ***<i>P</i><0.001.</p
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