The effect of <i>FZD6</i> siRNA on mRNA and protein expressions of FZD6, RhoA, Rac1 and JNK1 in IPEC-J2 cells.

<p>After 48 h of <i>FZD6</i> siRNA-1 (30nm, 50nm and 100nm) and negative control (100nm) treatment, the mRNA expressions of <i>FZD6</i> (A), <i>RhoA</i> (B), <i>Rac1</i> (C) and <i>JNK1</i> (D) in cells were analyzed by qPCR. The mRNA levels were normalized to the expression of GAPDH and plotted as relative mRNA expression. Western blot was performed to identify the protein levels of FZD6, RhoA, Rac1 and JNK1, and GAPDH was used as the control of sample loading (E and F). All results represented as mean ± SEM, n = 3. Values not sharing common letters are significantly different at <i>P</i> < 0.05.