FoxM1 mRNA level increases after Cdc7 depletion in HeLa and p53-negative HCT116.

<p>(<b>A</b>) HeLa cells were treated with indicated siRNAs for 24 hrs. FoxM1 (left), Plk1 (middle) and CyclinB1 (right) mRNA levels are presented. (<b>B</b>) Western analysis of the whole cell extracts of HeLa cells treated with indicated siRNAs for 48 hrs. A phosgel was used for the detection of MK2. Other proteins were separated on a 4–12% gradient gel. (<b>C</b>) The FoxM1 mRNA levels of HCT116 (p53-positive and -negative) cells treated with control or Cdc7 siRNA for 24 hrs. In A and C, mRNA levels were quantified by real time-PCR and the relative values normalized by the level of GAPDH mRNA are presented. (<b>D</b>) HeLa cells treated with indicated siRNAs for 48 hrs were fixed with 4% paraformaldehyde for 10 min and stained with anti-CyclinB1 antibody. Fractions of the cells showing nuclear localization of CyclinB1 are shown. Cdc7-D siRNA was used in these experiments.