miR-96 significantly decreases FOXO1 mRNA and protein levels in PCa cells.

<p><b>A</b>. Endogenous FOXO1 mRNA levels in PCa cell lines. mRNA levels were measured by qRT-PCR and normalized to the mean of GAPDH and PGK1. <b>B</b>. FOXO1 protein levels are significantly decreased in 22Rv1 cells (p=0.0065), LNCaP cells (p=0.0030), DU145 cells (p=0.0082) and VCaP cells (p=0.0096) upon miR-96 overexpression. The samples show biological triplicates and the FOXO1 protein levels were normalized to GAPDH protein levels. <b>C</b>. FOXO1 mRNA levels are significantly decreased in 22Rv1 cells after overexpression of miR-96(p=0.0341). Measured by qRT-PCR and normalized to the mean of GAPDH and PGK1. Error bars show standard error of mean.