CyclinB1 does not accumulate in cytoplasm in Cdc7-depleted U2OS cells.

<p>(<b>A</b>) U2OS cells expressing mKO2-CycinB1 were treated with siRNAs and time lapse images were recorded by LCV100. The time (hr) between the first appearance of cytosolic mKO2-CyclinB1 signal and its translocation into the nucleus was measured in the time lapse images of each cell population. In Cdc7-depleted U2OS, CyclinB1 does not accumulate in cytoplasm. However, co-depletion of Cdc7 and p53 caused CyclinB1 accumulation in cytoplasm for a longer period. The P-values of the two-tailed unpaired t-test were calculated by Prism software. (<b>B</b>) Western analysis of the whole cell extracts of U2OS cells treated with indicated siRNAs for 48 hrs. A phosgel was used for the detection of MK2. Other proteins were detected on a 4–12% gradient gel.